초록
<P><B>Abstract</B></P> <P>Mono and dual cultures of four white-rot basidiomycete species were evaluated for cellulase and xylanase activity under submerged fermentation conditions. Co-cultivation of <I>Pycnoporus coccineus</I> or <I>Trametes hirsuta</I> with <I>Schizophyllum commune</I> displayed antagonistic interactions resulting in the decrease of endoglucanase and total cellulase activities. In contrast, increases in cellulase and xylanase activity were revealed through the compatible interactions of <I>Irpex lacteus</I> with <I>S. commune</I>. Co-cultivation conditions were optimized for maximum enzyme production by <I>I. lacteus</I> and <I>S. commune</I>, the best producers of cellulase/xylanase and β-glucosidase, respectively. An optimized medium for the target enzyme production by the mixed culture was established in a laboratory fermenter yielding 7U/mL total cellulase, 142U/mL endoglucanase, 104U/mL xylanase, and 5.2U/mL β-glucosidase. The dual culture approach resulted in an enzymatic mixture with 11% improved lignocellulose saccharification potential compared to enzymes from a monoculture of <I>I. lacteus</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> White-rot basidiomycetes are promising sources of cellulases and xylanases. </LI> <LI> <I>Irpex lacteus</I> and <I>Schizophyllum commune</I> showed compatible interaction. </LI> <LI> Availability of carbon source determined outcomes of interspecific interaction. </LI> <LI> Co-culture conditions optimization improved hydrolases production. </LI> <LI> Synergistic enzyme preparation exhibited 11% higher hydrolysis of biomass. </LI> </UL> </P>