초록
<P>In an enzyme reaction using a gas substrate, the slow gas-liquid mass transfer rate of the gas substrate limits the total reaction rate, which causes low productivity. In this study, magnetic nanoparticles of cobalt ferrite silica (MSNs) and methyl-functionalized cobalt ferrite silica (methyl-MSNs) were synthesized and applied to a CO2-water system to evaluate gas-liquid mass transfer. The addition of methyl-MSNs increased the solubilized CO2 concentration by 31.1% compared to a 5.9% increase by MSNs. In an experiment where methyl-MSNs were added, the volumetric mass transfer coefficient was 78.3% higher than that in a control experiment without nanoparticles. The addition of methyl-MSNs in the formate dehydrogenase reaction resulted in a 12.0% increase in formic acid production and could decrease the reaction time required to finish the batch enzyme reaction from 1.5 h to 1.0 h. This result showed that the addition of methyl-MSNs could be useful for biological processes, including enzyme reactions, when using a gas substrate to improve productivity.</P>