초록
<P><B>Abstract</B></P> <P>Acetoin is useful as a food additive and a valuable intermediate chemicalwhich can be made from 2, 3-butanediol. To improve the production of acetoin, a compressed oxygen supply in a sealed aerated stirred tank reactor (COS-SSTR) based method is adopted. The strain of <I>Gluconobacter oxydans</I>NL71 was used for bio-oxidization of 2, 3-butanediol to acetoin. After optimization of the fed-batch concentration in an aerated stirred-tank reactor, the yield of acetoin was 97.6gL<SUP>−1</SUP>from an initial substrate concentration of 40gL<SUP>−1</SUP> 2, 3-butanediol. To improve the efficiency of acetoin production, we supplied COS-SSTR to obtain 165.9gL<SUP>−1</SUP>acetoin with a productivity of 11.43gL<SUP>−1</SUP> h<SUP>−1</SUP>in 36h. The product concentration and yield of the process are both new record for acetoin bioproduction. Our results demonstrate a promising industrial potential of the bacterial cell-catalysis of 2, 3-butanediol to acetoin in a significantly increased bioconversion rate.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Whole cell catalysis of 2, 3-butanediol to acetoin by <I>Gluconobacter Oxydans</I> NL71. </LI> <LI> Efficiently improving the productivity of acetoin using a compressed oxygen supply. </LI> <LI> The highest bioproduction of acetoin reported by fed-batch bioconversion. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>