초록
<P><B>Abstract</B></P> <P>We proposed a potential production platform of n-butanol in <I>Escherichia coli</I>. First, a butyrate-conversion strain was developed by removal of undesired genes and recruiting endogenous <I>atoDA</I> and <I>Clostridium adhE2</I>. Consequently, this <I>E. coli</I> strain grown on the M9 mineral salt with yeast extract (M9Y) was shown to produce 6.2g/L n-butanol from supplemented butyrate at 36h. The molar conversion yield of n-butanol on butyrate reaches 92%. Moreover, the production platform was advanced by additional inclusion of a butyrate-producing strain. This strain was equipped with a pathway comprising <I>atoDA</I> and heterologous genes for the synthesis of butyrate. Without butyrate, the butyrate-conversion and the butyrate-producing strains were co-cultured in M9Y medium and produced 5.5g/L n-butanol from glucose at 24h. The production yield on glucose accounts for 69% of the theoretical yield. Overall, it indicates a promise of the developed platform for n-butanol production in <I>E. coli</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Construct the redox-balanced pathway in the butyrate-conversion strain. </LI> <LI> Produce high n-butanol by the butyrate-conversion strain from supplemented butyrate. </LI> <LI> Couple the butyrate-producing and butyrate-conversion strains to establish the redox-balanced pathway of n-butanol. </LI> <LI> Produce high n-butanol from glucose by co-culturing of the butyrate- producing and butyrate-conversion strains. </LI> </UL> </P>