초록
<P><B>Abstract</B></P> <P>A novel and green approach for the coproduction of xylooligosaccharides (XOS), in terms of a series of oligosaccharide components from xylobiose to xylohexose, and fermentable sugars was developed using the prehydrolysis of acetic acid that was fully recyclable and environmentally friendly, followed by enzymatic hydrolysis. Compared to hydrochloric acid and sulfuric acid, acetic acid hydrolysis provided the highest XOS yield of 45.91% and the highest enzymatic hydrolysis yield. More than 91% conversion of cellulose was achieved in a batch-hydrolysis using only a cellulase loading of 20FPU/g cellulose and even a high solid loading of 20% without any special strategies. The acetic acid pretreated corncob should be washed adequately before saccharification to achieve complete hydrolysis. Consequently, a mass balance analysis showed that 139.8g XOS, 328.1g glucose, 25.1g cellobiose, and 147.8g xylose were produced from 1000g oven dried raw corncob.</P> <P><B>Highlights</B></P> <P> <UL> <LI> XOS and fermentable sugars were co-produced after novel prehydrolysis protocol. </LI> <LI> More than 91% cellulose conversion was achieved at high solid loading of 20%. </LI> <LI> The prehydrolyzed corncob cellulose was completely hydrolysable after rinse. </LI> <LI> 1000g corncob produced 139.8g XOS, 475.9g glucose and xylose, 25.1g cellobiose. </LI> </UL> </P>