BioChem - DOS

Scaling-up and ionic liquid-based extraction of pectinases from Aspergillus flavipes cultures

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Wolf-Má rquez, Vicente E.; Martí nez-Trujillo, M. Aurora; Aguilar Osorio, Guillermo; Patiñ o, Faustino; Á lvarez, Marí a S.; Rodrí guez, Ana; Sanromá n, M. Á ngeles; Deive, Francisco J.

<P><B>Abstract</B></P> <P>The viability of the scaling-up of pectinases production by <I>Aspergillus flavipes</I> at 5L-bioreactor scale has been demonstrated by keeping constant the power input, and a drastic increase in the endo- and exopectinolytic enzyme production was recorded (7- and 40-fold, respectively). The main process variables were modelled by means of logistic and Gompertz equations. In order to overcome the limitations of the conventional downstream strategies, a novel extraction strategy was proposed on the basis of the adequate salting-out potential of two biocompatible cholinium-based ionic liquids (N<SUB>1112OH</SUB>Cl and N<SUB>1112OH</SUB>H<SUB>2</SUB>PO<SUB>4</SUB>) in aqueous solutions of Tergitol, reaching more than 90% of extraction.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pectinase activity by <I>Aspergillus flavipes</I> was increased when scaled-up from 1 to 5L. </LI> <LI> Logistic, Gompertz and Luedeking &amp; Piret models were used to characterize the process. </LI> <LI> Cholinium-based ionic liquids are suitable extraction agents in tergitol solutions. </LI> <LI> Ionic liquid-based Aqueous Biphasic Systems led to 90% of pectinase extraction. </LI> </UL> </P>

2017

Elsevier

0960-8524

225

pp.326-335

10.1016/j.biortech.2016.11.067

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77089693

Pectinolytic enzymes; Ionic liquids; Bioreactor; Aqueous Biphasic Systems; Cholinium