초록
<P><B>Abstract</B></P> <P>In the study, the production of biohydrogen by extracted fermentation from sugar beet was evaluated. Effects of initial amount of sugar beet, biomass and particle size of sugar beet on biohydrogen formation were investigated. The hydrogen (H<SUB>2</SUB>) gas was predicted to be 78.6 mL at initial dry weight of sugar beet 24.6 g L<SUP>−1</SUP> and H<SUB>2</SUB> yield was calculated as 81.9 mLH<SUB>2</SUB> g<SUP>−1</SUP>TOC while biomass concentration (1 g L<SUP>−1</SUP>) and particle size (0.3 cm) were constant. The peak H<SUB>2</SUB> gas volume was predicted to be 139.9 mL at the low particle size of 0.1 cm. Hydrogen gas production potential was predicted as 143.6 mL h<SUP>−1</SUP>. The peak value of 197.9 mLH<SUB>2</SUB> g<SUP>−1</SUP>TOC was obtained with particle size of 0.1 cm when dry weight of sugar beet and initial amount of biomass was kept constant at 24.6 g L<SUP>−1</SUP> and 1 g L<SUP>−1</SUP>, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sugar beet was used as the raw material without pre-treatment methods. </LI> <LI> Extracted dark fermentation process enhanced utilization of sugar content. </LI> <LI> Extraction and fermentation process were carried out simultaneously. </LI> <LI> H<SUB>2</SUB> yield was 197.9 mLH<SUB>2</SUB> g<SUP>−1</SUP> TOC by providing high surface area. </LI> </UL> </P>