BioChem - DOS

Enhancement of ethanol production from glycerol in a Klebsiella pneumoniae mutant strain by the inactivation of lactate dehydrogenase

Process biochemistry

Oh, Baek-Rock; Seo, Jeong-Woo; Heo, Sun-Yeon; Hong, Won-Kyung; Luo, Lian Hua; Kim, Seonghun; Kwon, Ohsuk; Sohn, Jung-Hoon; Joe, Min-ho; Park, Don-Hee; Kim, Chul Ho

<P><B>Highlights</B></P><P>&#x025BA; We construed a lactate dehydrogenase gene defective mutant strain of <I>K. pneumoniae</I> GEM167 and expressed <I>Z. mobilis pdc</I> and <I>adhII</I> genes encoding pyruvate decarboxylase and aldehyde dehydrogenase, respectively, in the mutant strain. &#x025BA; Production level of ethanol from glycerol was remarkably improved by the genetic engineering upto 31.0g/l.</P> <P><B>Abstract</B></P><P>Previously, using &gamma;-irradiation treatment, we isolated a mutant strain of <I>Klebsiella pneumoniae</I> (named GEM167) that showed high-level ethanol production from glycerol. In the present study, in an effort to enhance ethanol production, we used a deletion of the lactate dehydrogenase gene to engineer a mutant strain incapable of lactate synthesis. In the &Delta;<I>ldhA</I> mutant of GEM167, the production of ethanol was significantly increased from 21.5g/l to 28.9g/l and from 0.93g/(lh) to 1.2g/(lh). Introduction of the <I>Zymomonas mobilis pdc</I> and <I>adhII</I> genes encoding pyruvate decarboxylase and aldehyde dehydrogenase, respectively, further improved the ethanol production level from glycerol to 31.0g/l; this is the highest level reported to date.</P>

2012

Elsevier

1359-5113

47

1

pp.156-159

10.1016/j.procbio.2011.10.011

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58161424

Klebsiella pneumoniae; Glycerol; Ethanol; Lactate dehydrogenase