초록
<P><B>Abstract</B></P> <P>The aim of this work was to study the effects of key trace elements (<I>i.e</I>., iron, copper and molybdenum) on the development of co-cultured n-damo and methanogenic bacteria consortium, which could realize <I>in situ</I> CH<SUB>4</SUB> production and utilization. The results showed that rational dosage, which was 50 mg/L of Fe, 1 mg/L of Cu and 5 mg/L of Mo, significantly stimulated the removal of NO<SUB>2</SUB> <SUP>−</SUP>. However, the activity of microbes was noticeably inhibited at 5 mg/L of Cu and 1 mg/L of Mo. Microbial community analysis indicated that the abundances of n-damo bacteria and methanogens showed a positive response to the rational dosage. Furthermore, the expression of key functional genes was enhanced under the rational dosage condition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Highest NO<SUB>2</SUB> <SUP>−</SUP> removal was obtained at dosage of 50 mg/L Fe, 1 mg/L Cu and 5 mg/L Mo. </LI> <LI> Fe was the key trace element for development of co-cultured bacteria consortium. </LI> <LI> Rational trace elements dosage stimulated bacterial growth rate. </LI> <LI> All trace element could facilitate expression of the related functional genes. </LI> </UL> </P>