초록
<P><B>Abstract</B></P> <P>The aim of the study was to evaluate a novel onsite enrichment approach to isolate a crude glycerol utilizing facultative anaerobic bacteria. An onsite enrichment in natural conditions resulted an isolate, <I>Lactobacillus brevis</I> N1E9.3.3, that can utilize glycerol and produce 1,3-propanediol with a yield of 0.89g<SUB>1,3-PDO</SUB>/g<SUB>G</SUB> <SUB>lycerol</SUB> and productivity of 0.78g<SUB>1,3-PDO</SUB>/l/h at pH-8.5 under anaerobic conditions. Batch fermentation experiments with glycerol–glucose co-fermentation strategy was carried out to evaluate the production of 1,3-propanediol and other byproducts. The effect of other carbon sources as co-substrate was also evaluated. At the optimized condition, 18.6g/l 1,3-propanediol was monitored when biodiesel industry generated crude glycerol and 2.5% glucose were used as the substrate.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Lactobacillus brevis</I> N1E9.3.3 was isolated specific onsite enrichment. </LI> <LI> Significant 1,3-propanediol production was observed with glucose as a co-substrate. </LI> <LI> Anaerobic batch cultivation at pH 8.5 resulted 0.89g<SUB>1,3-PDO</SUB>/g<SUB>Glycerol</SUB> yield. </LI> <LI> Co<SUP>+2</SUP> and vitamin B<SUB>12</SUB> was found to maximize 1,3-propanediol titers. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>