초록
<P>Pyruvate was produced from glucose by Escherichia coli BW25113 that contained formate dehydrogenase (FDH) from Mycobacterium vaccae. In aerobic shake-flask culture (K (L) a??=??4.9??min(-1)), the recombinant strain produced 6.7??g pyruvate??l(-1) after 24??h with 4??g sodium formate??l(-1) and a yield of 0.34??g pyruvate??g??glucose(-1). These values were higher than those of the original strain (0.2??g??l(-1) pyruvate and 0.02??g pyruvate??g??glucose(-1)). Based on the reaction mechanism of FDH, the introduction of FDH into E. coli enhances the accumulation of pyruvate by the regeneration of NADH from NAD(+) since NAD(+) is a shared cosubstrate with the pyruvate dehydrogenase complex, which decarboxylates pyruvate to acetyl-CoA and CO(2). The oxygenation level was enough high to inactivate lactate dehydrogenase, which was of benefit to pyruvate accumulation without lactate as a by-product.</P>