초록
<P><B>Abstract</B></P> <P>Biohydrogen production by the hyperthermophilic and halophilic bacterium <I>T. maritima</I>, using fruit and vegetable wastes as the carbon and energy sources was studied. Batch fermentation cultures showed that the use of a culture medium containing natural seawater and fruit and vegetable wastes can replace certain components (CaCl<SUB>2</SUB>, MgCl<SUB>2</SUB>, Balch's oligo-elements, yeast extract, KH<SUB>2</SUB>PO<SUB>4</SUB> and K<SUB>2</SUB>HPO<SUB>4</SUB>) present in basal medium. However, a source of nitrogen and sulfur remained necessary for biohydrogen production. When fruit and vegetable waste collected from a wholesale market landfill was used, no decreases in total H<SUB>2</SUB> production (139 mmol L<SUP>−1</SUP>) or H<SUB>2</SUB> yield (3.46 mol mol<SUP>−1</SUP>) was observed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> H2 production from fruit and vegetable wastes by <I>T. maritima</I> in seawater was studied. </LI> <LI> The supply of seawater can replace some components necessary for <I>T. maritima.</I> </LI> <LI> Using natural seawater medium recorded similar results to the mineral basal medium. </LI> <LI> No H<SUB>2</SUB> production by <I>T. maritima</I> in absence of nitrogen and sulfur sources. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>