초록
<P><B>Abstract</B></P> <P>This work explains the utilization of agro-waste (coconut and peanut shell) to produce mesoporous activated carbon which further utilized as a support material for lipase immobilization (<I>Candida antarctica</I> B, CALB). Various parameters affecting the binding of enzyme to activated carbon with high surface area (1603 m<SUP>2</SUP> g<SUP>−1</SUP>) were optimized. Maximum 200 μg g<SUP>−1</SUP> CALB has been loaded at 40 °C and pH 6.8 in 12 h by using glutaraldehyde as a cross-linker. The operational parameters such as pH (5.8–8.8) and temperature (30–70 °C) were optimized for free and immobilized form of lipase. In thermal stability (50–70 °C) study, immobilization of enzyme showed 2.35 folds increased half-life with respect to free enzyme. The samples, before and after immobilization, were characterized by specific surface area, FT-IR, SEM, XRD. This immobilized lipase was successfully used for the synthesis of cinnamyl acetate by transesterification reaction producing 94% conversion in 60 min. Catalytic efficiency (58 ± 1.08) was seen to be retained for more than five consecutive cycles of chemical reaction for repeated applications. Sequential results towards activity retention were obtained upto 30 days of storability study. In the context, this process constitutes a clean route for the development of sustainable biocatalysts from agro waste, capable of applications in various areas.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Utilization of activated carbon prepared from agro waste as a support material for immobilization of lipase CALB </LI> <LI> The immobilized lipase characterized by elemental analysis, surface properties, FT-IR, SEM and XRD. </LI> <LI> Improved thermal stability of immobilized lipase was expressed as half-life and deactivation energy. </LI> <LI> Reusability and storage stability was investigated to determine its industrial applicability. </LI> <LI> Immobilized biocatalyst was successfully used for synthesis of Cinnamyl acetate, a food flavor esters. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>