초록
Recombinant protein production under the control of the P<SUB>ADH3</SUB> was compared with Pichia pastoris P<SUB>AOX1</SUB> and P<SUB>GAP</SUB>. The single-copy-clones expressing Aspergillus niger xylanase (XylB) gene with the three different promoters were tested in shake flask and 5 L fed-batch fermentation processes. Recombinant protein production with P<SUB>ADH3</SUB>, P<SUB>AOX1</SUB> and P<SUB>GAP</SUB> were initiated by addition of ethanol, methanol and glucose, respectively in the culture medium. The fermentation process was carried out for 72 h at 30 <SUP>o</SUP>C, pH 5 and 30% dissolved oxygen. Extracellular protein production yield for P<SUB>ADH3</SUB> (3725 U/mL) was higher than for P<SUB>AOX1</SUB> (2095 U/mL) and P<SUB>GAP</SUB> (580 U/mL) at fermentor scale under the conditions tested. These results show that the P<SUB>ADH3</SUB> promoter is a promising tool for large scale production of recombinant proteins and can be an alternative to the P<SUB>AOX1</SUB> and P<SUB>GAP</SUB>.