초록
<P>Poly-3-<SMALL>D-</SMALL>hydroxybutyrate (PHB) is a promising biopolymer naturally produced by several bacterial species. In the present study, the robust baker’s yeast <I>Saccharomyces cerevisiae</I> was engineered to produce PHB from xylose, the main pentose found in lignocellulosic biomass. The PHB pathway genes from the well-characterized PHB producer <I>Cupriavidus necator</I> were introduced in recombinant <I>S. cerevisiae</I> strains already capable of pentose utilization by introduction of the fungal genes for xylose utilization from the yeast <I>Scheffersomyces stipitis</I>. PHB production from xylose was successfully demonstrated in shake-flasks experiments, with PHB yield of 1.17 ± 0.18 mg PHB g<SUP>−1</SUP> xylose. Under well-controlled fully aerobic conditions, a titer of 101.7 mg PHB L<SUP>−1</SUP> was reached within 48 hours, with a PHB yield of 1.99 ± 0.15 mg PHB g<SUP>−1</SUP> xylose, thereby demonstrating the potential of this host for PHB production from lignocellulose.</P>