초록
<P><B>Abstract</B></P><P><B>Aims</B></P><P>The aim of this research was to study the effect of time, temperature, sugar content and addition of diammonium phosphate (DAP) on ochratoxin A (OTA) removal by two strains of <I>Saccharomyces cerevisiae</I> using a completely randomized design.</P><P><B>Methods and Results</B></P><P>The strains were grown in a medium containing OTA (2？μg？l<SUP>−1</SUP>), two sugar levels (200 and 250？g？l<SUP>−1</SUP>), with or without DAP (300？mg？l<SUP>−1</SUP>), and incubated at 25–30°C. The yeasts were able to decrease the toxin amount by <I>c</I>. 70%, with the highest removing effect observed after 3？days at 30°C in the presence of 250？g？l<SUP>−1</SUP> of sugars and with DAP; after 10？days, the toxin was partially released into the medium. The strains produced high ethanol and glycerol contents, showed high tolerance to single/combined stress conditions and possessed β‐<SMALL>d</SMALL>‐glucosidase, pectinase and xylanase activities.</P><P><B>Conclusions</B></P><P>Ochratoxin A removal was affected by time, temperature, sugar and addition of DAP. Moreover, the phenomenon was reversible.</P><P><B>Significance and Impact of the Study</B></P><P>Ochratoxin A removal could be an interesting trait for the selection of promising strains; however, the strains removing efficiently the toxin could release it back; thus, the selection of the starter should take into account both the removal and the binding ability of OTA.</P>