초록
<P><B>Abstract</B></P> <P>Development of cost effective and highly efficient process for bio-based succinic acid (SA) production is a main concern for industry. The metabolically engineered <I>Y. lipolytica</I> strain PGC01003 was successfully used for SA production with high titre. However, this strain possesses as main drawback with a low growth rate when glycerol is used as a feedstock. Herein, gene <I>GUT1,</I> encoding glycerol kinase, was overexpressed in strain PGC01003 with the aim to improve glycerol uptake capacity. In the resulting strain RIY420, glycerol uptake was 13.5% higher than for the parental strain. <I>GUT1</I> gene overexpression also positively influences SA production. In batch bioreactor, SA titre, yield and productivity were 32%, 39% and 143% higher, respectively, than for the parental strain PGC01003. Using a glycerol feeding strategy, SA titre, yield and productivity were further improved by 11%, 5% and 10%, respectively. Moreover, the process duration to yield the highest concentration of SA in the culture supernatant was reduced by 9%. This demonstrated the contribution of metabolically engineered strain RIY420 to lower SA process cost and increase the efficiency of bio-based SA production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Low productivity caused expensive production cost of biobased succinic acid (SA). </LI> <LI> High productivity contributes to reduction in process cost. </LI> <LI> Increasing glycerol uptake rate and SA productivity by overexpression of gene <I>GUT1.</I> </LI> <LI> The process duration to yield the highest concentration of SA was reduced by 9%. </LI> <LI> Overexpression strain developed cost effective and efficient SA production process. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>