초록
<P>Simultaneous saccharification and fermentation (SSF) experiments involving water hyacinth (<I>Eichhornia crassipes</I>), an abundantly available renewable bioresource, using hydrolytic enzymes, and fermentative microbes were investigated. Water hyacinth containing 30.01 (%, w/w) cellulose, 44.49 (%, w/w) hemicellulose, and 20.04 (%, w/w) lignin was subjected to three different pretreatments, namely, wet oxidation, phosphoric acid (H<SUB>3</SUB>PO<SUB>4</SUB>)‐acetone, and ammonia fiber explosion (AFEX). Hydrolytic enzymes, namely, recombinant <I>Clostridium thermocellum</I> cellulase (GH5) and hemicellulase (GH43), <I>Trichoderma reesei</I> and <I>Bacillus subtilis</I> AS3 cellulases were employed separately for saccharification. <I>Saccharomyces cerevisiae</I> and <I>Candida shehatae</I> were used for fermentation. The AFEX pretreated 1% (w/v) water hyacinth along with recombinant cellulase (GH5)‐hemicellulase (GH43) consortium gave the highest ethanol titer of 1.52 g/L as compared with wet oxidation (1.23 g/L) and phosphoric acid‐acetone pretreatments (1.31 g/L). The best SSF combination with 5% (w/v) substrate at shake flask contributed an ethanol titer and yield of 7.83 g/L, 0.266 (g of ethanol/g of substrate) and its scale up at bioreactor level resulted in significantly higher ethanol titer and yield of 14.39 g/L and 0.489 (g/g), respectively. 93.0 (%, v/v) ethanol from bioreactor was recovered by rotary evaporator with 20.4% purification efficiency. © 2013 American Institute of Chemical Engineers Environ Prog, 33: 1396–1404, 2014</P>