초록
<P><B>Highlights</B></P><P>► Three native strains of <I>Botryococcus braunii</I> isolated from the Kolleru lake, Andhra Pradesh, India. ► Strains identified based on the morphological and molecular characterization. ► Strains AP103, AP104 and AP105 evaluated for biomass, lipid and hydrocarbon production. ► Lipid rich AP103 strain was selected and mass culture under open raceway pond. ► Under open raceway pond strain AP103 produced 1.8gL<SUP>−1</SUP> of biomass, 19% lipids, and 11% hydrocarbons.</P> <P><B>Abstract</B></P><P>Three different strains of colonial green alga <I>Botryococcus bruanii</I> Kutz. AP103, AP104 and AP105 were isolated from the freshwater lake Kolleru, Andhra Pradesh, India. The morphological features of these three isolates were studied under laboratory conditions. Molecular identification of the strains was carried out using 18S rRNA analysis and their systematic position was confirmed to species level as <I>B. braunii</I>. Among these isolates, <I>B. braunii</I> AP103 recorded highest biomass concentration of 1.7±0.12gL<SUP>−1</SUP>, 17% lipids, 17% proteins, 32% carbohydrates and 13% hydrocarbons under laboratory condition. Under open raceway pond AP103 produced a biomass concentration of 1.8±0.13gL<SUP>−1</SUP>, 19% lipids, 33% carbohydrates, 18% proteins and 11% hydrocarbons. The hydrocarbons profile showed the presence of heptadecane (34%) and hexadecane (12.5%). Oleic (25.7%), linolenic (34.26%) and palmitic (9.42%) acids were the major fatty acids present in the lipids extracted from AP103.</P>