BioChem - DOS

Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Salvachú a, Davinia; Smith, Holly; St. John, Peter C.; Mohagheghi, Ali; Peterson, Darren J.; Black, Brenna A.; Dowe, Nancy; Beckham, Gregg T.

<P><B>Abstract</B></P> <P>The production of chemicals alongside fuels will be essential to enhance the feasibility of lignocellulosic biorefineries. Succinic acid (SA), a naturally occurring C4-diacid, is a primary intermediate of the tricarboxylic acid cycle and a promising building block chemical that has received significant industrial attention. <I>Basfia succiniciproducens</I> is a relatively unexplored SA-producing bacterium with advantageous features such as broad substrate utilization, genetic tractability, and facultative anaerobic metabolism. Here <I>B. succiniciproducens</I> is evaluated in high xylose-content hydrolysates from corn stover and different synthetic media in batch fermentation. SA titers in hydrolysate at an initial sugar concentration of 60g/L reached up to 30g/L, with metabolic yields of 0.69g/g, and an overall productivity of 0.43g/L/h. These results demonstrate that <I>B. succiniciproducens</I> may be an attractive platform organism for bio-SA production from biomass hydrolysates.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This is the first report of <I>B. succiniciproducens</I> performance in hydrolysate. </LI> <LI> Succinic acid yields are higher in biomass hydrolysates than pure sugars. </LI> <LI> The side product lactic acid decreases significantly when using biomass hydrolysate. </LI> <LI> The bacterium detoxifies furfural and HMF to furfuryl- and HMF-alcohol. </LI> <LI> A stoichiometric model was utilized to analyze the side product distributions. </LI> </UL> </P>

2016

Elsevier

publisher-specific-oa

0960-8524

214

pp.558-566

10.1016/j.biortech.2016.05.018

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75722569

http://manuscript.elsevier.com/S0960852416306587/pdf/S0960852416306587.pdf

Biorefinery; Biochemical; Corn stover; Xylose; Actinobacillus succinogenes