초록
<P><B>Abstract</B></P> <P>Enzymatic fuel cells have received considerable attention because of their potential for direct conversion of abundant raw materials such as cellulose to electricity. The use of multi-enzyme cascades is particularly attractive as they offer the possibility of achieving a series of complex reactions at higher efficiencies. Here we reported the use of a DNA-guided approach to assemble a five-component enzyme cascade for direct conversion of cellulose to gluconic acid and H<SUB>2</SUB>O<SUB>2</SUB>. Site-specific co-localization of β-glucosidase and glucose oxidase resulted in over 11-fold improvement in H<SUB>2</SUB>O<SUB>2</SUB> production from cellobiose, highlighting the benefit of substrate channeling. Although a more modest 1.5-fold improvement in H<SUB>2</SUB>O<SUB>2</SUB> production was observed using a five-enzyme cascade, due to H<SUB>2</SUB>O<SUB>2</SUB> inhibition on enzyme activity, these results demonstrated the possibility to enhance the production of gluconic acid and H<SUB>2</SUB>O<SUB>2</SUB> directly from cellulose by DNA-guided enzyme assembly.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Direct conversion of cellulose to gluconic acid and H<SUB>2</SUB>O<SUB>2</SUB> using a DNA-guided five-enzyme cascade. </LI> <LI> Enhanced H<SUB>2</SUB>O<SUB>2</SUB> production by over 11-fold using the assembled BlgA and GOX cascade. </LI> <LI> Enhanced H<SUB>2</SUB>O<SUB>2</SUB> production using the assembled five-enzyme cascade. </LI> </UL> </P>