BioChem - DOS

Genome mining of astaxanthin biosynthetic genes from Sphingomonas sp. ATCC 55669 for heterologous overproduction in Escherichia coli

Biotechnology journal

Ma, Tian; Zhou, Yuanjie; Li, Xiaowei; Zhu, Fayin; Cheng, Yongbo; Liu, Yi; Deng, Zixin; Liu, Tiangang

<P><B>Abstract</B></P><P>As a highly valued keto&#x2010;carotenoid, astaxanthin is widely used in nutritional supplements and pharmaceuticals. Therefore, the demand for biosynthetic astaxanthin and improved efficiency of astaxanthin biosynthesis has driven the investigation of metabolic engineering of native astaxanthin producers and heterologous hosts. However, microbial resources for astaxanthin are limited. In this study, we found that the &alpha;&#x2010;Proteobacterium <I>Sphingomonas</I> sp. ATCC 55669 could produce astaxanthin naturally. We used whole&#x2010;genome sequencing to identify the astaxanthin biosynthetic pathway using a combined PacBio&#x2010;Illumina approach. The putative astaxanthin biosynthetic pathway in <I>Sphingomonas</I> sp. ATCC 55669 was predicted. For further confirmation, a high&#x2010;efficiency targeted engineering carotenoid synthesis platform was constructed in <I>E. coli </I> for identifying the functional roles of candidate genes. All genes involved in astaxanthin biosynthesis showed discrete distributions on the chromosome. Moreover, the overexpression of exogenous <I>E. coli </I> idi in Sphingomonas sp. ATCC 55669 increased astaxanthin production by 5.4&#x2010;fold. This study described a new astaxanthin producer and provided more biosynthesis components for bioengineering of astaxanthin in the future.</P>

2016

WILEY&#x2010;VCH Verlag

1860-6768

1860-7314

11

2

pp.228-237

10.1002/biot.201400827

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