초록
Most biomass pretreatment processes for sugar production are run at low-solid concentration (<10wt.%). Subcritical carbon dioxide (CO<SUB>2</SUB>) could provide a more sustainable pretreatment medium while using relative high-solid contents (15wt.%). The effects of subcritical CO<SUB>2</SUB> pretreatment of sugarcane bagasse to the solid and glucan recoveries at different pretreatment conditions were investigated. Subsequently, enzymatic hydrolysis at different hydrolysis time was applied to obtain maximal glucose yield, which can be used for ethanol fermentation. The maximum glucose yield in enzyme hydrolyzate reached 38.5g based on 100g raw material after 72h of enzymatic hydrolysis, representing 93.0% glucose in sugarcane bagasse. The enhanced digestibilities of subcritical CO<SUB>2</SUB> pretreated sugarcane bagasse were due to the removal of hemicellulose, which were confirmed by XRD, FTIR, SEM, and TGA analyses.