BioChem - DOS

Repeated ethanol production from sweet sorghum juice concentrated by membrane separation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Sasaki, Kengo; Tsuge, Yota; Sasaki, Daisuke; Kawaguchi, Hideo; Sazuka, Takashi; Ogino, Chiaki; Kondo, Akihiko

<P><B>Abstract</B></P> <P>Sequential batch fermentation from sweet sorghum juice concentrated by membrane separation (ultrafiltration permeation and nanofiltration concentration) to increase sugar contents, was investigated. Ethanol production at 5th batch fermentation by <I>Saccharomyces cerevisiae</I> BY4741 attained 113.7&plusmn;3.1gL<SUP>&minus;1</SUP> (89.1&plusmn;2.2% of the theoretical ethanol yield) from 270.0&plusmn;22.6gL<SUP>&minus;1</SUP> sugars, corresponding to 98.7% of ethanol titer attained at the 1st batch fermentation. This titer was comparable to ethanol production of 115.8&plusmn;0.6gL<SUP>&minus;1</SUP> (87.1&plusmn;2.7% of the theoretical ethanol yield) obtained at 5th batch fermentation with 3gL<SUP>&minus;1</SUP> yeast extract and 6gL<SUP>&minus;1</SUP> polypeptone. Increase of cell density in the concentrated sweet sorghum juice was observed during sequential batch fermentation, as indicated by increased OD<SUB>600</SUB>. Utilization of sweet sorghum juice as the sole source, membrane separation, and <I>S. cerevisiae</I> was a cost-effective process for high ethanol production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sweet sorghum juice was concentrated by membrane separation. </LI> <LI> Sequential batch fermentation was done with concentrated juice. </LI> <LI> Ethanol was repeatedly produced with high levels by <I>Saccharomyces cerevisiae</I>. </LI> <LI> Exogenous nutrient sources were not necessary. </LI> <LI> Growth of <I>S. cerevisiae</I> was observed in concentrated juice. </LI> </UL> </P>

2015

Elsevier

0960-8524

186

pp.351-355

10.1016/j.biortech.2015.03.127

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72026958

Sweet sorghum juice; Bioethanol; Sequential batch fermentation; Nanofiltration; Saccharomyces cerevisiae