BioChem - DOS

New improved method for fructooligosaccharides production by Aureobasidium pullulans

Carbohydrate polymers

Dominguez, A.; Nobre, C.; Rodrigues, L.R.; Peres, A.M.; Torres, D.; Rocha, I.; Lima, N.; Teixeira, J.

Fructooligosaccharides are prebiotics with numerous health benefits within which the improvement of gut microbiota balance can be highlighted, playing a key role in individual health. In this study, an integrated one-stage method for FOS production via sucrose fermentation by Aureobasidium pullulans was developed and optimized using experimental design tools. Optimization of temperature and agitation speed for maximizing the FOS production was performed using response surface methodology. Temperature was found to be the most significant parameter. The optimum fermentation conditions were found to be 32<SUP>o</SUP>C and 385rpm. Under these conditions, the model predicted a total FOS production yield of 64.7g<SUB>FOS</SUB>/g<SUB>sucrose</SUB>. The model was validated at optimal conditions in order to check its adequacy and accuracy and an experimental yield of 64.1 (+/-0.0)g<SUB>FOS</SUB>/g<SUB>sucrose</SUB> was obtained. A significant improvement of the total FOS production yields by A. pullulans using a one-stage process was obtained.

2012

Applied Science Publishers ; Elsevier Science Ltd

0144-8617

89

4

pp.1174-1179

10.1016/j.carbpol.2012.03.091

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63205982

https://repositorium.sdum.uminho.pt/bitstream/1822/19935/1/dominguez_2012.pdf

Fructooligosaccharides; Aureobasidium pullulans; Transfructosylation; One-stage fermentation process; Response surface methodology; Product yield