초록
<P><B>Abstract</B></P> <P>In the present work, production of hydrogen <I>via</I> dark fermentation has been carried out using the hydrolyzed rice straw and <I>Clostridium pasteurianum</I> (MTCC116). The hydrolysis reaction of 1.0% alkali pretreated rice straw was performed at 70°C and 10% substrate loading <I>via</I> Fe<SUB>3</SUB>O<SUB>4</SUB>/Alginate nanocomposite (Fe<SUB>3</SUB>O<SUB>4</SUB>/Alginate NCs) treated thermostable crude cellulase enzyme following the previously established method. It is noticed that under the optimized conditions, at 70°C the Fe<SUB>3</SUB>O<SUB>4</SUB>/Alginate NCs treated cellulase has produced around 54.18g/L sugars as the rice straw hydrolyzate. Moreover, the efficiency of the process illustrates that using this hydrolyzate, <I>Clostridium pasteurianum</I> (MTCC116) could produce cumulative hydrogen of 2580ml/L in 144h with the maximum production rate of 23.96ml/L/h in 96h. In addition, maximum dry bacterial biomass of 1.02g/L and 1.51g/L was recorded after 96h and 144h, respectively with corresponding initial pH of 6.6 and 3.8, suggesting higher hydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Nanomaterial treated thermostable crude cellulase used for hydrolysis of rice straw. </LI> <LI> Working temperature of enzymatic hydrolysis is 70°C using waste biomass. </LI> <LI> High released sugar was used for hydrogen production <I>via</I> dark fermentation. </LI> <LI> Cumulative hydrogen of 2580ml/L was recorded from 54.18g/L sugars in 144h. </LI> <LI> Maximum hydrogen production rate of 23.96ml/L/h was recorded in 96h. </LI> </UL> </P>