Downscale fermentation for xylooligosaccharides production by recombinant Bacillus subtilis 3610
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
기타
논문
Downscale fermentation for xylooligosaccharides production by recombinant Bacillus subtilis 3610
학술지
Carbohydrate polymers
저자명
Amorim, Clá udia; Silvé rio, Sara C.; Gonç alves, Raquel F.S.; Pinheiro, Ana C.; Silva, Soraia; Coelho, Elisabete; Coimbra, Manuel A.; Prather, Kristala L.J.; Rodrigues, Lí gia R.
초록
<P><B>Abstract</B></P> <P>The global demand of prebiotics such as xylooligosaccharides (XOS) has been growing over the years, motivating the search for different production processes with increased efficiency. In this study, a cloned <I>Bacillus subtilis</I> 3610, containing the xylanase gene xyn2 of <I>Trichoderma reesei</I> coupled with an endogenous secretion tag, was selected for XOS production through direct fermentation of beechwood xylan. A mixture of XOS with a degree of polymerization ranging from 4 to 6 was obtained, presenting high stability after a static <I>in vitro</I> digestion (98.5 ± 0.2%). The maximum production yield expressed as total XOS per amount of xylan (306 ± 4 mg/g) was achieved after 8 h of fermentation operating under one-time impulse fed-batch. The optimal conditions found were pH 6.0 and 42.5 °C, using 2.5 g/L of initial concentration of xylan increased up to 5.0 g/L at 3 h. Xylopentaose was the major oligosaccharide produced, representing 47% of the total production yield.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Beechwood xylan was used to produce XOS using <I>B. subtilis</I> 3610 wild type and clones. </LI> <LI> The clone selected contained a secretion tag coupled to <I>T. reesei xyn</I>2 gene. </LI> <LI> The XOS obtained were (1→4)-Xyl with DP 4–6. </LI> <LI> One-time impulse fed-batch mode increased the XOS maximum production at 8 h. </LI> <LI> Optimal conditions: 2.5 g/L xylan increased up to 5.0 g/L at 3 h, pH 6.0 and 43 °C. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>