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Immobilization of α-amylase and amyloglucosidase onto ion-exchange resin beads and hydrolysis of natural starch at high concentration

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논문

Immobilization of α-amylase and amyloglucosidase onto ion-exchange resin beads and hydrolysis of natural starch at high concentration

학술지

Bioprocess and biosystems engineering

저자명

Gupta, Kapish; Jana, Asim Kumar; Kumar, Sandeep; Maiti, Mithu

초록

<P>α-Amylase was immobilized on Dowex MAC-3 with 88 % yield and amyloglucosidase on Amberlite IRA-400 ion-exchange resin beads with 54 % yield by adsorption process. Immobilized enzymes were characterized to measure the kinetic parameters and optimal operational parameters. Optimum substrate concentration and temperature were higher for immobilized enzymes. The thermal stability of the enzymes enhanced after the immobilization. Immobilized enzymes were used in the hydrolysis of the natural starch at high concentration (35 % w/v). The time required for liquefaction of starch to 10 dextrose equivalent (DE) and saccharification of liquefied starch to 96 DE increased. Immobilized enzymes showed the potential for use in starch hydrolysis as done in industry.</P>

발행연도

2013

발행기관

Springer-Verlag

ISSN

1615-7591

ISSN

1615-7605

36

11

페이지

pp.1715-1724

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1 2023-12-11

논문; 2013-04-10

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