초록
<P><B>Abstract</B></P> <P>Hydrogen production by immobilized <I>Enterobacter aerogenes</I> on functionalized multi-walled carbon nanotube (MWCNT-COOH) in repeated batch mode was studied. Fourier transform infrared (FTIR) spectroscopy and field emission scanning electron microscopy (FESEM) were employed to confirm immobilization of <I>E. aerogenes</I> successfully. The effect of MWCNT-COOH concentrations (0.2, 0.6, and 1.2 mg/mL) on hydrogen production was investigated. The present study showed that immobilized <I>E. aerogenes</I> on 1.2 mg/mL MWCNT-COOH resulted in higher hydrogen yield (2.2 moL/mol glucose), hydrogen production rate (2.72 L/L.h), and glucose degradation efficiency (96.20%) and shorter the lag phase (1 h) compared to the free <I>E. aerogenes</I>. Modified Gompertz and Logistic models were employed to predict the cumulative hydrogen production successfully.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Immobilization of <I>E. aerogenes</I> on MWCNT was used to improve hydrogen production. </LI> <LI> Immobilization of <I>E. aerogenes</I> on MWCNT reduces lag phase and increases HY and HPR. </LI> <LI> Modified Gompertz and Logistic models were used to predict hydrogen production. </LI> </UL> </P>