BioChem - DOS

Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using Cupriavidus necator

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

da Cruz Pradella, José Geraldo; Ienczak, Jaciane Lutz; Delgado, Cecí lia Romero; Taciro, Marilda Keico

<P>Poly(3-hydroxybutyrate) (PHB) biosynthesis from soybean oil by Cupriavidus necator was studied using a bench scale bioreactor. The highest cell concentration (83 g l(-1)) was achieved using soybean oil at 40 g l(-1) and a pulse of the same concentration. The PHB content was 81% (w/w), PHB productivity was 2.5 g l(-1) h(-1), and the calculated Y(p/s) value was 0.85 g g(-1). Growth limitation and the onset of PHB biosynthesis took place due to exhaustion of P, and probably also Cu, Ca, and Fe.</P>

2012

Springer-Verlag

0141-5492

1573-6776

34

6

pp.1003-1007

10.1007/s10529-012-0863-1

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART62273728