BioChem - DOS

Enhanced production of erythritol by Yarrowia lipolytica on glycerol in repeated batch cultures

Journal of industrial microbiology & biotechnology

Miroń czuk, Aleksandra M.; Furgała, Joanna; Rakicka, Magdalena; Rymowicz, Waldemar

<P>Erythritol is an important natural sweetener, industrially produced only by fermentation on glucose media. Glycerol is an important renewable feedstock as it is the major by-product of the biodiesel production process; here we present an alternative way to convert this low-cost substrate into value-added products, such as erythritol. Repeated batch cultures (RBC) were performed to improve the productivity of erythritol from pure and crude glycerol. An acetate negative mutant of <I>Yarrowia lipolytica</I> Wratislavia K1 was found to be applicable for the production of high amounts of erythritol in RBC. When 20&nbsp;% of fresh replaced medium was added, the strain Wratislavia K1 was able to produce 220&nbsp;g&nbsp;l <SUP>&#x2212;1</SUP> erythritol, which corresponded to a 0.43&nbsp;g&nbsp;g<SUP>&#x2212;1</SUP> yield and a productivity of 0.54&nbsp;g&nbsp;l<SUP>&#x2212;1</SUP>&nbsp;h<SUP>&#x2212;1</SUP>. Additionally, the activity of the culture remained stable for more than 1,000&nbsp;h, i.e., 11 cycles of the repeated batch bioreactors.</P>

2014

Springer Berlin Heidelberg

1367-5435

1476-5535

41

1

pp.57-64

10.1007/s10295-013-1380-5

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68402302

Erythritol; Yarrowia lipolytica; Pure glycerol; Crude glycerol; Repeated batch culture