초록
The interplay among the culture parameters, light intensity and illumination conditions, mechanical mixing conditions, and reactor configurations during O<SUB>2</SUB> evolution and H<SUB>2</SUB> production in sulfur-deprived Chlamydomonas reinhardtii cultures is studied in this work. The advective-diffusive reaction equation is used to describe the diffusion and the local biochemical reactions of the species involved in the biological process for H<SUB>2</SUB> production. Solutions of the diffusion equation are obtained for different boundary conditions. An empirical equation is used to describe the relation between the light attenuation coefficient and the initial chlorophyll concentration, which characterizes both the culture absorption property and the mechanical mixing condition. The dynamics of O<SUB>2</SUB> evolution and H<SUB>2</SUB> production in a tubular photobioreactor illuminated under three different light conditions on the boundary is studied for the cultures with different initial chlorophyll concentrations. For the culture with the initial chlorophyll concentration of 18 mg/L, the light attenuation level of K<SUB>a</SUB><SUP>0</SUP> = 0.125, and the reference light intensity of 236.3 μE m<SUP>-2</SUP> s<SUP>-1</SUP>, the results indicate that the maximum H<SUB>2</SUB> production is 166 ml for the tubular reactor under uniform light illumination, 163 ml for the flat plate reactor under two-side light illumination, 85 and 75 ml for the tubular and the flat plate reactors, respectively, under one-side light illumination.