초록
<P><B>Abstract</B></P> <P>Sterol profiles were obtained from cyanobacteria <I>Phormidium autumnale</I>, cultivated in a heterotrophic system using three distinct sources of carbon: glucose, sucrose, and agroindustrial slaughterhouse wastewater. A simultaneous saponification-extraction ultrasound-assisted method was performed to determine sterol and other non-saponified compounds in the dry biomasses. A total of 24 compounds were observed in the biomasses, including hope-22,29-en-3-one, squalene, and 22 other sterols. Using wastewater as a carbon source, the microalgae biomass produced a diversity of sterols such as stigmasterol (455.3 μg g<SUP>−1</SUP>) and β-sitosterol (279.0 μg g<SUP>−1</SUP>). However, with glucose it is possible to produce ergosterol (1033.3 μg g<SUP>−1</SUP>). Squalene was found in all the cultures, with 1440.4 μg g<SUP>−1</SUP>, 225.4 μg g<SUP>−1</SUP>, and 425.6 μg g<SUP>−1</SUP> for glucose, sucrose, and slaughterhouse wastewater biomasses, respectively. Several intermediate compounds from those sterols were found. These data provide the construction of the sterol metabolism according to the literature for <I>P. autumnale</I> heterotrophically cultured.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>P autumnale</I> produced distinct sterols from different exogenous carbon source. </LI> <LI> Squalene was a sterol precursor found in all biomasses. </LI> <LI> Cholesterol, β-sitosterol and stigmasterol were main sterol produced by <I>P autumnale.</I> </LI> <LI> High amounts of ergosterol was produced in the glucose culture. </LI> <LI> Several sterol pathways' intermediary metabolites were identified in <I>P autumnale.</I> </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>