초록
<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P>Efficient conversion of inhibitor containing pentose rich stream into bioethanol or biogas is still challenging due to several technological challenges; however, this stream can be efficiently assimilated into lipids by oleaginous yeasts such as <I>Rhodotorula</I>. In this work, a continuous and nonsterile process was developed for bioconversion of waste streams into lipids for biodiesel using growth‐inhibitor‐tolerant oleaginous yeast.</P><P><B>RESULTS</B></P><P>An inhibitor‐tolerating property of the strain was used to develop a cyclic and continuous near axenic cultivation process in a 2 L nonsterile bioreactor to enhance valorization of waste streams and to reduce energy requirements for media and vessel sterilization. After media optimization, continuous cultivation resulted in significant increase in biomass (14.90 g L<SUP>−1</SUP> day<SUP>−1</SUP>) and lipid productivity (6.15 g L<SUP>−1</SUP> day<SUP>−1</SUP>) as compared to flask (biomass ∼3 g L<SUP>−1</SUP> day<SUP>−1</SUP> and lipid productivity ∼0.6 g L<SUP>−1</SUP> day<SUP>−1</SUP>) results. Palmitic acid (C16:0) and oleic acid (C18:1) were the major fatty acids, accounting for almost 80% of total lipids, thus making lipid produced in the process suitable for biodiesel production.</P><P><B>CONCLUSION</B></P><P>Inhibitors present in waste streams successfully maintained near axenic conditions during the cultivation. Maximum dilution rate of 1 was achieved after media optimization, which resulted in complete valorization of nutrients into biomass and lipids with high productivity. © 2018 Society of Chemical Industry</P>