초록
<P><B>Abstract</B></P> <P>This study adopted five pretreatment means (base, aeration, γ-radiation, acid and heat-shock) for enriching hydrogen-producing bacteria from anaerobically digested sludge, aiming to investigate the microbial community diversity during fermentative hydrogen production using various pretreatments as inoculum. The experimental results indicated that all five pretreatments could effectively enrich hydrogen-producing bacteria from the seed sludge, while the microbial communities showed a great difference among various pretreated groups. The most three dominant genera were <I>Paraclostridium</I> (28.6%), <I>Clostridium sensu stricto</I> 1 (19.8%) and <I>Terrisporobacter</I> (19.4%) for base pretreated group, <I>Enterococcus</I> (67.2%), <I>Clostridium sensu stricto</I> 1 (10%) and <I>Citrobacter</I> (5.6%) for aeration pretreated group, <I>Clostridium sensu stricto</I> 1 (63.9%), <I>Paeniclostridium</I> (9.3%) and <I>Romboutsia</I> (7%) for γ-radiation pretreated group, <I>Clostridium sensu stricto</I> 1 (51.9%), <I>Romboutsia</I> (22.4%) and <I>Paeniclostridium</I> (8.2%) for acid pretreated group, and <I>Paraclostridium</I> (61.2%), <I>Exiguobacterium</I> (23.1%) and <I>Clostridium sensu stricto</I> 1 (8.1%) for heat-shock pretreated group, respectively. Different microbial communities resulted in diverse process performance and metabolic pathway. Heat-shock pretreatment achieved the maximum hydrogen yield of 1.58 mol/mol-glucose and maximum hydrogen production rate of 37.65 mL/h. The dominance of genus <I>Paraclostridium</I> was supposed to be responsible for the highest hydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Five pretreatment means were used for enriching hydrogen-producing bacteria. </LI> <LI> The microbial community diversity was analyzed during hydrogen fermentation process. </LI> <LI> Various pretreatments had significant influence on microbial community diversity. </LI> <LI> Microbial community affected the process performance and metabolic pathway. </LI> <LI> The dominance of <I>Paraclostridium</I> contributed to the higher hydrogen production. </LI> </UL> </P>