초록
<P><B>Abstract</B></P> <P>Furfuralcohol (FOL) is an important intermediate for the production of lysine, ascorbic acid, and lubricants. It can be used as a hypergolic fuel in rocketry. In this study, it was attempted to synthesize FOL from xylose by tandem catalysis with solid acid SO<SUB>4</SUB> <SUP>2−</SUP>/SnO<SUB>2</SUB>-Montmorillonite and recombination <I>Escherichia coli</I> CCZU-K14 whole cells. Using SO<SUB>4</SUB> <SUP>2−</SUP>/SnO<SUB>2</SUB>-Montmorillonite (3.0wt% dosage) as catalyst, a highest furfural yield of 41.9% was achieved from xylose at 170°C for 20min. Furthermore, <I>Escherichia coli</I> CCZU-K14 whole cells were used for bioconverting furfural to FOL. The optimum biocatalytic reaction temperature, reaction pH, cosubstrate concentration, and substrate concentration were 30°C, 6.5, 1.5mol glucose/mol furfural, and 200mM, respectively. Finally, the yield of FOL from 200mM furfural was achieved to 100% by <I>Escherichia coli</I> CCZU-K14 whole cells after 24h. In conclusion, this strategy show high potential application for the effective synthesis of FOL.</P> <P><B>Highlights</B></P> <P> <UL> <LI> One-pot synthesis of furfuralcohol was conducted by chemo-enzymatic catalysis. </LI> <LI> Conversion of xylose to furfural with SO<SUB>4</SUB> <SUP>2−</SUP>/SnO<SUB>2</SUB>-MMT was optimized. </LI> <LI> Bioreduction of furfural to furfuralcohol with <I>E. coli</I> CCZU-K14 was optimized. </LI> <LI> Furfural liquor showed biocompatibility towards <I>E. coli</I> CCZU-K14 whole cells. </LI> <LI> Biosynthesis of furfuralcohol from 200mM furfural was obtained at 100% yield. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>