Biosynthesis of benzoylformic acid from benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1 in toluene-water biphasic system

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- 바이오플라스틱
  1. 플라스틱
  2. 기타
- 바이오정밀화학
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  2. 화학제품
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- 의료용 화학소재
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논문

Biosynthesis of benzoylformic acid from benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1 in toluene-water biphasic system

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

He, Yu-Cai; Zhou, Qiong; Ma, Cui-Luan; Cai, Zhi-Qiang; Wang, Li-Qun; Zhao, Xi-Yue; Chen, Qun; Gao, Da-Zhou; Zheng, Ming; Wang, Xu-Dong; Sun, Quan

초록

AbstractBenzoylformic acid was synthesized from the hydrolysis of benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1. In this study, an aqueous–toluene biphasic system was developed for highly efficient production of benzoylformic acid from the hydrolysis of benzoyl cyanide. In the aqueous–toluene biphasic system, the phase volume ratio, buffer pH and reaction temperature were optimized. Using fed-batch method, a total of 932mM benzoylformic acid accumulated in the reaction mixture after the 10th feed. Moreover, enzymatic hydrolysis of benzoyl cyanide using calcium alginate entrapped resting cells was carried out in the aqueous–toluene biphasic system, and efficient biocatalyst recycling was achieved as a result of cell immobilization in calcium alginate, with a product-to-biocatalyst ratio of 14.26g benzoylformic acid g−1 dry cell weight (DCW) cell after 20 cycles of repeated use.

발행연도

2012

ISSN

0960-8524

권

115

페이지

pp.88-95

DOI

10.1016/j.biortech.2011.09.084

상세정보링크

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63191443