초록
<P><B>Abstract</B></P> <P>The production of 1,3-propanediol (1,3-PDO) from glucose was investigated using <I>Klebsiella pneumoniae</I> J2B, which converts glycerol to 1,3-PDO and synthesize an essential coenzyme B<SUB>12</SUB>. In order to connect the glycolytic pathway with the pathway of 1,3-PDO synthesis from glycerol, i.e., to directly produce diol from glucose, glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate phosphatase from <I>Saccharomyces cerevisiae</I> were overexpressed. Additionally, the effect of expression levels and the use of isoforms of these two enzymes on glycerol and 1,3-PDO production were studied. Furthermore, to prevent loss of produced glycerol, the glycerol oxidation pathways were disrupted. Finally, the conversion rate of glycerol to 1,3-PDO was increased via homologous overexpression of glycerol dehydratase and 1,3-PDO oxidoreductase. The resultant strain successfully produced 1,3-PDO from glucose at a yield of 0.27mol/mol along with glycerol at 0.52mol/mol. Improvement of the engineered <I>K. pneumoniae</I> J2B to further increase conversion of glycerol to 1,3-PDO is discussed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>K. pneumoniae</I> J2B feasibility for 1,3-PDO production from glucose was studied. </LI> <LI> <I>K. pneumoniae</I> J2B was engineered and overexpressed GPD, GPP, GDHt and DhaT. </LI> <LI> The recombinant strain produced 1,3-PDO from glucose, but with low yield. </LI> <LI> CCR, byproducts accumulation and NADH unavailability reduced 1,3-PDO production. </LI> </UL> </P>