BioChem - DOS

Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae

Green chemistry : an international journal and green chemistry resource : GC

Zhang, Lijie; Liu, Qiuyuan; Ge, Yongsheng; Li, Lixiang; Gao, Chao; Xu, Ping; Ma, Cuiqing

<P>Acetoin (AC) is regarded as one of the top potential sugar-derived chemical building blocks that can be used as food additives, precursors in chemical synthesis, and plant growth promoting molecules. In this study, a low-cost lignocellulosic resource of pretreated corn stover was used as a carbon source to produce AC. After redirecting the metabolic flux, fine tuning reducing power, and eliminating carbon catabolite repression in <I>Enterobacter cloacae</I> SDM, a systematically engineered strain SDM 53 was constructed, which is able to utilize glucose and xylose efficiently and simultaneously. Using fed-batch fermentation of SDM 53, 45.6 g L<SUP>&minus;1</SUP> AC was produced at a rate of 1.52 g L<SUP>&minus;1</SUP> h<SUP>&minus;1</SUP> using the lignocellulosic hydrolysate. Biotechnological synthesis of AC has various advantages such as being sustainable and environment-friendly. With its desirable properties, the engineered strain SDM 53 may be a potential choice for the industrial production of AC.</P><BR><BR><P>Graphic Abstract</P><P>Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered <I>Enterobacter cloacae</I>.<BR><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5gc01638j'><BR></P>

2016

The Royal Society of Chemistry

1463-9262

1463-9270

18

6

pp.1560-1570

10.1039/c5gc01638j

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75180106