BioChem - DOS

Butanol production from alkali-pretreated rice straw by co-culture of Clostridium thermocellum and Clostridium saccharoperbutylacetonicum

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Kiyoshi, Keiji; Furukawa, Masataka; Seyama, Tomoko; Kadokura, Toshimori; Nakazato, Atsumi; Nakayama, Shunichi

<P><B>Abstract</B></P> <P>The co-culture of cellulolytic <I>Clostridium thermocellum</I> NBRC 103400 and butanol-producing <I>Clostridium saccharoperbutylacetonicum</I> strain N1-4 produced 5.5g/L of butanol from 40g/L of delignified rice straw pretreated with 1% (wt/vol) NaOH. The addition of cellulase (100U/gbiomass) in a co-culture system significantly increased butanol production to 6.9g/L using 40g/L of delignified rice straw. Compared to the control, this increase in butanol production was attributed to the enhancement of exoglucanase activity on lignocellulose degradation in experimental samples. The results showed that the co-culture system in conjunction with enhanced exoglucanase activity resulted in cost-effective butanol production from delignified rice straw.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Alkali pretreatment significantly reduced lignin content of rice straw up to 76%. </LI> <LI> Co-culture of cellulolytic and butanol producing <I>Clostridium</I> species was applied. </LI> <LI> The co-culture system produced 5.5g/L of butanol from delignified rice straw. </LI> <LI> Addition of cellulase enhanced butanol titer (6.9g/L) from delignified rice straw. </LI> <LI> Enhancement of exoglucanase is important for butanol production from rice straw. </LI> </UL> </P>

2015

Elsevier

0960-8524

186

pp.325-328

10.1016/j.biortech.2015.03.061

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72026936

Clostridium species; Butanol production; Lignocellulose; Delignification; Co-culture