초록
This study evaluated the potential of a microbial consortium collected from a pulp and paper mill wastewater treatment plant (WWTP) for converting cellulose to hydrogen, biogas and organic acids. Fermentation tests were conducted in batch reactors fed with different concentrations of cellulose as substrate: (C1) 2.0 g L -1; (C2) 5.0 g L -1 and (C3) 10.0 g L -1. The parameters investigated were hydrogen, biogas, organic acids, carbohydrates and pH. The maximum hydrogen production was 14.77, 39.25 and 22.53 mmol L -1, and the maximum methane was 4.40, 3.72 and 9.56 mmol L -1, for C1, C2 and C3, respectively. Butyric acid was the main metabolite generated, with maximum concentrations of 2.2, 1.8 and 2.2 g L -1 for C1, C2 and C3, respectively. The decrease in hydrogen production was accompanied by the production of methane, acetic acid and hydrogen sulfide in the three tests, probably related to hydrogenotrophic methanogenesis, homoacetogenesis and sulfidogenesis, respectively. The phylogenetic characterization of the bacterial community was performed by cloning and sequencing analysis. The microorganisms identified in the consortium were similar (> 95%) to Clostridium sp., Klebsiella sp., Routella sp. and Desulfovibrio sp. These genera were associated with hydrogen production, degradation of cellulosic substrates, and/or hydrogen-consuming microorganisms.