BioChem - DOS

Extraction of lipase from Rhodotorula glutinis fermentation culture by aqueous two-phase partitioning

Fluid phase equilibria

Khayati, G.; Alizadeh, S.

The effects of molecular weight of polyethylene glycol (PEG), various salts and broth loading on the partitioning of lipase from cell free fermentation broth of Rhodotorula glutinis were studied in PEG/salts aqueous two-phase systems at pH 6.6 and 24<SUP>o</SUP>C. With the application of 17.5 (%, w/w) PEG4000/12.5 (%, w/w) oxalate potassium and 12.5 (%, v/w) fermentation broth loading was found most appropriate since high lipase purification factor of 13.9, and yield of enzyme in top phase 71.2% were obtained. The value of K<SUB>e</SUB> decreases as the PEG molecular weight increases.

2013

Elsevier Scientific Pub. Co

0378-3812

353

pp.132-134

10.1016/j.fluid.2013.05.037

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66422968

Lipase; Aqueous two-phase system; Rhodotorula glutinis