초록
<P>Sugarcane bagasse was chemically modified by polyethylenimine (PEI) and glutaraldehyde (GA) and then used as a support to immobilize Clostridium acetobutylicum XY16 in the process of butanol production. Compared with batch fermentation using unmodified sugarcane bagasse, 22.3?g/L total solvents were produced by cells immobilized on 4?g/L PEI treated sugarcane bagasse with high solvent productivity of 0.62?g/(L?h) and glucose consumption rate of 1.67?g/(L?h). Improvement of 14, 43, and 37?% in total solvent titer, solvent productivity and glucose consumption rate was observed, respectively. Enhanced solvent production of 25.14?g/L was obtained when using a high concentration of glucose of 80?g/L. Continuous fermentation was studied using PEI/GA modified sugarcane bagasse as immobilization support with a range of dilution which rates from 0.2 to 2.5 to find an optimal condition. The maximum solvent productivity of 11.32?g/(L?h) was obtained at a high dilution rate of 2.0?h(-1).</P>