BioChem - DOS

Effect of the nitrogen source on the hydrogen production metabolism and hydrogenases of Clostridium butyricum CWBI1009

International journal of hydrogen energy

Hamilton, Christopher; Calusinska, Magdalena; Baptiste, Stacy; Masset, Julien; Beckers, Laurent; Thonart, Philippe; Hiligsmann, Serge

<P><B>Abstract</B></P> <P>Investigations were carried out to determine the effect of various concentrations of organic and ammonium nitrogen sources on fermentative hydrogen production by the strain <I>Clostridium butyricum</I> CWBI1009. The results indicated that the H<SUB>2</SUB>-producing metabolism of the strain is favoured within the range (0.56&ndash;0.062 g<SUB>N</SUB>/L) of peptone and (NH<SUB>4</SUB>)<SUB>2</SUB>SO<SUB>4</SUB>. Optimal overall performance (<I>i.e.</I> 1.43 &plusmn; 0.08 mol H<SUB>2</SUB>/mol glucose and 1.08 &plusmn; 0.03 mL<SUB>H2</SUB>/h, respectively) was achieved with 0.062 g<SUB>N</SUB>/L of casein peptone. The study of the amino acid uptake and the gene expression pattern for four [FeFe]-hydrogenases and the nitrogenase showed that nitrogen was in excess in all the experiments with a nitrogen concentration above 0.062 g<SUB>N</SUB>/L and, at that optimal concentration, the expression of the <I>HydB2</I> gene would be responsible for the much higher H<SUB>2</SUB> yield.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effect of nitrogen sources was studied on <I>Clostridium</I> biohydrogen production. </LI> <LI> Four organic or ammonium nitrogen sources and ten concentrations were tested. </LI> <LI> Optimal overall performance was achieved with 0.062 g<SUB>N</SUB>/L of casein peptone. </LI> <LI> H<SUB>2</SUB> production yield and rate peaked at 1.43 mol<SUB>H2</SUB>/mol<SUB>glucose</SUB> and 1.08 mL<SUB>H2</SUB>/h. </LI> <LI> Amino acid distribution and hydrogenase expression were investigated. </LI> </UL> </P>

2018

Elsevier

0360-3199

1879-3487

43

11

pp.5451-5462

10.1016/j.ijhydene.2017.12.162

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART81413333

Biohydrogen; Clostridium; Nitrogen; Hydrogenase; Amino acid