초록
<P><B>Highlights</B></P><P>► On-site enzyme production is a potential strategy for sustainable cellulosic ethanol. ► Use of low value carbon source for microbial growth is an effective strategy. ► The Amazon rainforest <I>Trichoderma harzianum</I> is a useful candidate for on-site enzyme production.</P> <P><B>Abstract</B></P><P>The on-site production of cellulases is an important strategy for the development of sustainable second-generation ethanol production processes. This study concerns the use of a specific cellulolytic enzyme complex for hydrolysis of pretreated sugar cane bagasse. Glycosyl hydrolases (FPase, xylanase, and β-glucosidase) were produced using a new strain of <I>Trichoderma harzianum</I>, isolated from the Amazon rainforest and cultivated under different conditions. The influence of the carbon source was first investigated using shake-flask cultures. Selected carbon sources were then further studied under different pH conditions using a stirred tank bioreactor. Enzymatic activities up to 121FPU/g, 8000IU/g, and 1730IU/g of delignified steam-exploded bagasse+sucrose were achieved for cellulase, xylanase and β-glucosidase, respectively. This enzymatic complex was used to hydrolyze pretreated sugar cane bagasse. A comparative evaluation, using an enzymatic extract from <I>Trichoderma reesei</I> RUTC30, indicated similar performance of the <I>T. harzianum</I> enzyme complex, being a potential candidate for on-site production of enzymes.</P>