초록
<P><B>Abstract</B></P> <P>In this work, multiple reuses of <I>Rhodococcus ruber</I> TH3 free cells for the hydration of acrylonitrile to produce acrylamide in a membrane dispersion microreactor were carried out. Through using a centrifuge, the reactions reached 39.9, 39.5, 38.6 and 38.0wt% of the final acrylamide product concentration respectively within 35min in a four cycle reuse of free cells. In contrast, using a stirring tank, free cells could only be used once with the same addition speed of acrylonitrile with a microreactor. Through observing the dissolution behavior of acrylonitrile microdroplets in a free cell solution using a coaxial microfluidic device and microscope, it was found that the acrylonitrile microdroplets with a diameter of 75μm were rarely observed within a length of 2cm channel within 10s, which illustrated that the microreactor can intensify the reaction rate to reduce the inhibition of acrylonitrile and acrylamide.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Multiple reuses of <I>R. ruber</I> TH3 free cells were achieved in microreactor. </LI> <LI> Product concentration reached 39.92–38.04wt% within 35min in four cycle reuse. </LI> <LI> The dissolution behavior of acrylonitrile microdroplets was observed. </LI> <LI> Any inhibition on the biocatalyst caused by acrylonitrile can be ignored. </LI> </UL> </P>