Assessment of β-carotene content, cell physiology and morphology of the yellow yeast Rhodotorula glutinis mutant 400A15 using flow cytometry

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- 화장품용 기능성소재
  1. 기능성
  2. 계면활성제⁄증점제
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논문

Assessment of β-carotene content, cell physiology and morphology of the yellow yeast Rhodotorula glutinis mutant 400A15 using flow cytometry

학술지

Journal of industrial microbiology & biotechnology

저자명

Cutzu, Raffaela; Clemente, Ana; Reis, Alberto; Nobre, Beatriz; Mannazzu, Ilaria; Roseiro, José Lopes da Silva, Teresa

초록

AbstractFlow cytometry was used to assess β-carotene content, cell membrane permeability, cell size and granularity in Rhodotorula glutinis mutant 400A15 grown under different oxygen transfer coefficients (k L a) and carbon to nitrogen ratios (C/N). A Doehlert distribution was used in order to select the best conditions that induced the highest carotenoids production. The highest β-carotene content (0.79 mg g−1 DCW) at the lowest k L a and C/N (5 × 10−3 s−1 and 11.3 respectively). Under these conditions, the biomass concentration attained 18.60 g L−1. The highest ratio of cells with permeabilised membranes (2.6 %), and the highest cell size and granularity were also obtained under these conditions. It was observed that C/N showed a stronger influence than the k L a on the measured cell parameters.

발행연도

2013

발행기관

Oxford University Press

ISSN

1367-5435

ISSN

1476-5535

권

호

페이지

pp.865-875