초록
<P><B>Abstract</B></P> <P>The conversion of ricinoleic acid from renewable sources to long-chain α,ω-dicarboxylic acids or w-hydroxyl carboxylic acids by microbial processes is constrained by toxicity issues. Here, we demonstrate the possible role of <I>Corynebacterium glutamicum</I> as a new microbial strategy for the biotransformation of fatty acids. The established strain <I>Escherichia coli</I> failed to grow at 5 mM <I>n</I>-heptanoic acid, while the specific growth rate of <I>C. glutamicum</I> declined by 28%. We partially constructed a previously designed multistep biocatalytic pathway in <I>C. glutamicum,</I> and confirmed that the <I>C. glutamicum</I> biocatalyst successfully converted ricinoleic acid to undec-9-enoic acid, heptyl ester via 12-keto-oleic acid. We investigated the effects of cultivation and reaction temperatures, and the type and concentration of non-ionic detergent on recombinant <I>C. glutamicum</I> whole-cell bioconversion. At a cultivation temperature of 30 °C and a reaction temperature of 35 °C, and in the presence of 0.09 g/L Triton X-100, the whole-cell <I>C. glutamicum</I> biocatalyst produced 0.8 mM undec-9-enoic acid, heptyl ester from 1.9 mM 12-ketooleic acid. It also generated 0.7 mM undec-9-enoic acid, heptyl ester from 5.5 mM ricinoleic acid. This is the first report of undec-9-enoic acid, heptyl ester production using a recombinant <I>C. glutamicum</I>-based biocatalyst.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>C. glutamicum</I> ATCC13032 has high tolerance to <I>n</I>-heptanoic acid. </LI> <LI> <I>C. glutamicum</I> biocatalyst produces 0.7 mM of undec-9-enoic acid, heptyl ester from renewable fatty acid. </LI> <LI> This work may help to provide potential of new microbial platform strain for the biotransformation of fatty acid. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>