BioChem - DOS

Effects of pH and dissolved CO2 level on simultaneous production of 2,3-butanediol and succinic acid using Klebsiella pneumoniae

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Cheng, K.K.; Wu, J.; Wang, G.Y.; Li, W.Y.; Feng, J.; Zhang, J.A.

The influences of pH and dissolved CO<SUB>2</SUB> level on the regulation of growth and formation of catabolic end products have been investigated in Klebsiella pneumoniae. With increasing CO<SUB>2</SUB> levels, there were no apparent changes in 2,3-butanediol production but succinic acid productions were enhanced significantly. A novel strategy for co-production of 2,3-butanediol and succinic acid using K. pneumoniae was developed by controlling pH and dissolved CO<SUB>2</SUB> concentration in fermentation medium. Under the optimum condition, maximal 77.1gl<SUP>-1</SUP> 2,3-butanediol and 28.7gl<SUP>-1</SUP> succinic acid were obtained after 60h of fed-batch fermentation, giving a 2,3-butanediol+succinic acid yield of 1.03molmol<SUP>-1</SUP> glucose. This type of fermentation producing two commercial interests at the same fermentation process might be considered for a promising biological production process which will decrease the production cost by sharing the operation and recovery cost.

2013

Elsevier Applied Science

0960-8524

135

pp.500-503

10.1016/j.biortech.2012.08.100

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65836494

2,3-Butanediol; Fermentation; Glucose; Klebsiella pneumoniae; Succinic acid