초록
<P><B>Abstract</B></P> <P>Oxygen supply is an important factor during <I>Crypthecodinium cohnii</I> fermentation for docosahexaenoic acid (DHA) production. However, few studies about the intrinsic correlation between dissolved oxygen (DO) and cellular metabolism have been reported. In this study, the responses of <I>C. cohnii</I> to different DO levels were evaluated. The results showed the growth and glucose consumption rates of <I>C. cohnii</I> were much higher under high oxygen supply condition. Furthermore, GC-MS based comparative metabolomic analysis was employed to discriminate the responsive metabolites associated with varying DO levels. The results showed the intermediates involved in glycolytic pathway and TCA cycle were up-regulated under high DO levels at exponential phase. At stationary phase, under high DO levels, metabolites involved in triacylglycerol metabolism were up-regulated, while the OPP pathway intermediate product ribose 5-phosphate was down-regulated. Together, these results provide useful insights into the functional metabolic relationship between DO levels and DHA production in <I>C. cohnii</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Growth characteristics of <I>C. cohnii</I> under different oxygen supply evaluated. </LI> <LI> Effects of different oxygen supply at molecular level revealed for <I>C. cohnii.</I> </LI> <LI> Potential biomarkers in response to different oxygen supplies identified in <I>C. cohnii.</I> </LI> </UL> </P>